CD247 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CD247 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA10499A0RB
• Supplier Catalog Number: CSB-PA10499A0Rb
• Alternative Catalog Number: CSB-PA10499A0RB-100UG, CSB-PA10499A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: 4930549J05Rik antibody, A430104F18Rik antibody, AW552088 antibody, CD247 antibody, CD247 antigen antibody, Cd247 molecule antibody, Cd3 antibody, CD3 antigen, zeta polypeptide, isoform CRA_b antibody, CD3 antigen, zeta subunit antibody, CD3-eta antibody, CD3H antibody, CD3Q antibody, Cd3z antibody, CD3Z antigen zeta polypeptide (TiT3 complex) antibody, CD3Z_HUMAN antibody, CD3zeta antibody, CD3zeta chain antibody, MGC140430 antibody, T cell receptor T3 zeta chain antibody, T cell surface glycoprotein CD3 zeta chain antibody, T-cell antigen receptor complex, zeta subunit of CD3 antibody, T-cell receptor T3 zeta chain antibody, T-cell surface glycoprotein CD3 zeta chain antibody, T3z antibody, TCR zeta chain antibody, TCRk antibody, TCRZ antibody, TCRzeta antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P20963
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CD247

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Images

Immunofluorescent analysis of HepG2 cells using CSB-PA10499A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA10499A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA10499A0Rb at dilution of 1:100

Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: CD247 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa

Immunoprecipitating CD247 in jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA10499A0Rb in jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA10499A0Rb (8µg) + jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)