CD9 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA10559A0RB
Article Name: CD9 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA10559A0RB
Supplier Catalog Number: CSB-PA10559A0Rb
Alternative Catalog Number: CSB-PA10559A0RB-100UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: Tetraspanin 29 antibody, 5H9 antibody, 5H9 antigen antibody, Antigen defined by monoclonal antibody 602 29 antibody, Antigen defined by monoclonal antibody 60229 antibody, BA-2/p24 antigen antibody, BA2 antibody, BTCC 1 antibody, BTCC1 antibody, CD9 antibody, CD9 antigen antibody, CD9 antigen p24 antibody, CD9 molecule antibody, CD9_HUMAN antibody, Cell growth inhibiting gene 2 protein antibody, Cell growth-inhibiting gene 2 protein antibody, DRAP 27 antibody, DRAP27 antibody, GIG2 antibody, Growth inhibiting gene 2 protein antibody, Leukocyte antigen MIC3 antibody, MIC3 antibody, Motility related protein antibody, Motility-related protein antibody, MRP 1 antibody, MRP-1 antibody, MRP1 antibody, p24 antibody, p24 antigen antibody, Tetraspanin-29 antibody, Tspan 29 antibody, Tspan-29 antibody, TSPAN29 antibody
Clonality: Polyclonal
UniProt: P21926
Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20C, Avoid freeze / thaw cycles.
Purity: Antigen Affinity Purified
Form: Liquid
Target: CD9
Application Dilute: Recommended dilution: WB:1:500-1:3000, IHC:1:20-1:200
IHC image of CSB-PA10559A0Rb diluted at 1:50 and staining in paraffin-embedded human small tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA10559A0Rb diluted at 1:50 and staining in paraffin-embedded human small bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA10559A0Rb diluted at 1:50 and staining in paraffin-embedded human small endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA10559A0Rb diluted at 1:50 and staining in paraffin-embedded human small prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), L02 whole cell lysate(30µg), A549 whole cell lysate(30µg), K562 whole cell lysate(30µg)
All lanes: CD9 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 23-30 kDa
Observed band size: 23 kDa
Exposure time:120s