KRT18 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: KRT18 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA10629A0RB
• Supplier Catalog Number: CSB-PA10629A0Rb
• Alternative Catalog Number: CSB-PA10629A0RB-100UG, CSB-PA10629A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Zebrafish
• Conjugation: Unconjugated
• Alternative Names: Cell proliferation inducing gene 46 protein antibody, Cell proliferation inducing protein 46 antibody, Cell proliferation-inducing gene 46 protein antibody, CK 18 antibody, CK-18 antibody, CK18 antibody, CYK 18 antibody, CYK18 antibody, Cytokeratin 18 antibody, Cytokeratin endo B antibody, Cytokeratin-18 antibody, K 18 antibody, K18 antibody, K1C18_HUMAN antibody, KA18 antibody, Keratin 18 antibody, Keratin 18, type I antibody, Keratin D antibody, keratin, type I cytoskeletal 18 antibody, Keratin-18 antibody, Krt18 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P05783
• Buffer: Preservative: 0.1mol/L NaCl, 15mmol/L NaN3, PH7.2
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: KRT18

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:50-1:500

Images

Western blot
All lanes: KRT18 antibody at 2µg/ml
Lane 1: K562 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

Western blot
All lanes: KRT18 antibody at 12µg/ml
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

Immunofluorescent analysis of HepG2 cells using CSB-PA10629A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IHC image of CSB-PA10629A0Rb diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate
All lanes: KRT18 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

Immunocytochemistry analysis of human placenta tissue using CSB-PA10629A0Rb at dilution of 1:100