AMH Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: AMH Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA12639A0RB
• Supplier Catalog Number: CSB-PA12639A0Rb
• Alternative Catalog Number: CSB-PA12639A0RB-100UG, CSB-PA12639A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: AMH antibody, MIF antibody, Muellerian-inhibiting factor antibody, Anti-Muellerian hormone antibody, AMH antibody, Muellerian-inhibiting substance antibody, MIS antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P03971
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: AMH

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1500, IF:1:50-1:200

Images

Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA12639A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA12639A0Rb at dilution of 1:100

Immunofluorescent analysis of MCF-7 cells using CSB-PA12639A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IHC image of CSB-PA12639A0Rb diluted at 1:1400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: AMH antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa