CASP7 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CASP7 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA15479A0RB
• Supplier Catalog Number: CSB-PA15479A0Rb
• Alternative Catalog Number: CSB-PA15479A0RB-100UG, CSB-PA15479A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: apoptosis-related cysteine peptidase antibody, Apoptotic protease Mch-3 antibody, CASP-7 antibody, CASP7 antibody, CASP7_HUMAN antibody, Caspase-7 subunit p11 antibody, CMH-1 antibody, ICE-LAP3 antibody, ICE-like apoptotic protease 3 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P55210
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CASP7

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000

Images

Western blot
All lanes: CASP7 antibody at 2µg/ml
Lane 1: A549 whole cell lysate
Lane 2: HGC-27 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 35, 29, 38, 32 kDa
Observed band size: 35 kDa

Immunofluorescence staining of MCF-7 cells with CSB-PA15479A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA15479A0Rb diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA15479A0Rb diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating CASP7 in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA15479A0Rb in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA15479A0Rb (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)