Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form:
liquid
Target:
MTOR
Application Dilute:
WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200
Immunofluorescence staining of methanol-fixed MCF cells using mTOR(Phospho-Ser2448) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR(Phospho-Ser2448) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis ofparaffin-embedded human breast carcinomatissue using mTOR (Phospho-Ser2448) Antibody
Western blot analysis of extracts from Hela cells, treatedwith IFNa or calf intestinal phosphatase (CIP), using mTOR(Phospho-Ser2448) Antibody.
Western blot analysis of extracts from 293 cells untreatedor treated with PMA using mTOR(Phospho-Ser2448) Antibody.
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