SMAD1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SMAD1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA618998LA01HU
• Supplier Catalog Number: CSB-PA618998LA01HU
• Alternative Catalog Number: CSB-PA618998LA01HU-100UG, CSB-PA618998LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: BSP-1 antibody, BSP1 antibody, HsMAD1 antibody, JV4-1 antibody, JV41 antibody, MAD homolog 1 antibody, MAD mothers against decapentaplegic homolog 1 antibody, Mad related protein 1 antibody, Mad-related protein 1 antibody, MADH1 antibody, MADR1 antibody, Mothers against decapentaplegic homolog 1 antibody, Mothers against DPP homolog 1 antibody, SMA- AND MAD-RELATED PROTEIN 1 antibody, SMAD 1 antibody, SMAD family member 1 antibody, SMAD mothers against DPP homolog 1 antibody, Smad1 antibody, SMAD1_HUMAN antibody, TGF beta signaling protein 1 antibody, Transforming growth factor-beta-signaling protein 1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q15797
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: SMAD1

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:100-1:1000, IF:1:200-1:500

Images

Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA618998LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA618998LA01HU at dilution of 1:100

Immunofluorescence staining of Hela cells with CSB-PA618998LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA618998LA01HU diluted at 1:600 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate
All lanes: SMAD1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53, 16 kDa
Observed band size: 53 kDa