CHRNA2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CHRNA2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA621886ESR1HU
• Supplier Catalog Number: CSB-PA621886ESR1HU
• Alternative Catalog Number: CSB-PA621886ESR1HU-100UL, CSB-PA621886ESR1HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: ACHA2_HUMAN antibody, AChR antibody, Cholinergic receptor nicotinic alpha 2 antibody, Chrna2 antibody, Neuronal acetylcholine receptor protein subunit alpha 2 antibody, Neuronal acetylcholine receptor subunit alpha-2 antibody, Nicotinic Acetylcholine Receptor alpha 2 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q15822
• Buffer: Preservative: 0.03% Proclin 300<br /> Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: CHRNA2

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200,IF:1:20-1:200

Images

Immunohistochemistry of par

Immunofluorescence staining of PC-3 cell with CSB-PA621886ESR1HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry image of CSB-PA621886ESR1HU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: HL-60 whole cell lysate(20µg), Hela whole cell lysate(20µg), Jurkat whole cell lysate(20µg), PC-3 whole cell lysate(20µg)
All lanes: CHRNA2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 59 kDa
Observed band size: 60 kDa
Exposure time: 300s