RPE65 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA624103LA01HU
Article Name: RPE65 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA624103LA01HU
Supplier Catalog Number: CSB-PA624103LA01HU
Alternative Catalog Number: CSB-PA624103LA01HU-100UG, CSB-PA624103LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human, Rat
Conjugation: Unconjugated
Alternative Names: All-trans-retinyl-palmitate hydrolase antibody, LCA 2 antibody, LCA2 antibody, Leber congenital amaurosis antibody, mRPE 65 antibody, mRPE65 antibody, p63 antibody, rd 12 antibody, rd12 antibody, Retinal pigment epithelium specific 61 kDa protein antibody, Retinal pigment epithelium specific 65 kDa protein antibody, Retinal pigment epithelium specific protein antibody, Retinal pigment epithelium specific protein 65kDa antibody, Retinal pigment epithelium-specific 65 kDa protein antibody, Retinitis pigmentosa 20 antibody, Retinoid isomerohydrolase antibody, Retinol isomerase antibody, RP 20 antibody, RP20 antibody, RPE 65 antibody, RPE65 antibody, RPE65_HUMAN antibody, sRPE 65 antibody, sRPE65 antibody
Clonality: Polyclonal
UniProt: Q16518
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: RPE65
Application Dilute: Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500
Immu
Immunofluorescence staining of Hela cell with CSB-PA624103LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC-3 cell with CSB-PA624103LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC-3 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: PC-3 whole cell lysate, MCF7 whole cell lysate, Rat Brain tissue lysate
All lanes: RPE65 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 65 kDa
Observed band size: 61 kDa