ITPRIPL2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA664748LA01HU
Article Name: ITPRIPL2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA664748LA01HU
Supplier Catalog Number: CSB-PA664748LA01HU
Alternative Catalog Number: CSB-PA664748LA01HU-100UL, CSB-PA664748LA01HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: ITPRIPL2 antibody, Inositol 1,4,5-trisphosphate receptor-interacting protein-like 2 antibody
Clonality: Polyclonal
UniProt: Q3MIP1
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purity: Antigen Affinity Purified
Form: Liquid
Target: ITPRIPL2
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IF:1:20-1:100
Immunofluorescence staining of Hela cell with CSB-PA664748LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cell with CSB-PA664748LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), A549 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), U251 whole cell lysate(20µg), JK whole cell lysate(20µg)
All lanes: ITPRIPL2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 10s