GLE1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GLE1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA700651EA01HU
• Supplier Catalog Number: CSB-PA700651EA01HU
• Alternative Catalog Number: CSB-PA700651EA01HU-100UG, CSB-PA700651EA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: GLE 1 antibody, GLE1 antibody, GLE1 like protein antibody, GLE1 like RNA export mediator antibody, GLE1 RNA export mediator homolog antibody, GLE1 RNA export mediator like (yeast) antibody, GLE1-like protein antibody, GLE1_HUMAN antibody, GLE1L antibody, hGLE1 antibody, LCCS 1 antibody, LCCS antibody, LCCS1 antibody, Nucleoporin GLE1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q53GS7
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: GLE1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Images

Immunofluorescent analysis of MCF-7 cells using CSB-PA700651EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunocytochemistry analysis of human melanoma using CSB-PA700651EA01HU at dilution of 1:100

Immunocytochemistry analysis of human breast cancer using CSB-PA700651EA01HU at dilution of 1:100

Western Blot
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate
All lanes: GLE1 antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 80, 76 kDa
Observed band size: 80 kDa

Immunoprecipitating GLE1 in A549 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA700651EA01HU in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA700651EA01HU (6µg) + A549 whole cell lysate (500µg)
Lane 3: A549 whole cell lysate (20µg)