MEST Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MEST Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA704858ESR1HU
• Supplier Catalog Number: CSB-PA704858ESR1HU
• Alternative Catalog Number: CSB-PA704858ESR1HU-100UL, CSB-PA704858ESR1HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: DKFZp686L18234 antibody, EC 3.-.-.- antibody, Mesoderm specific transcript (mouse) homolog antibody, Mesoderm specific transcript homolog (mouse) antibody, Mesoderm-specific transcript homolog protein antibody, MEST antibody, MEST_HUMAN antibody, MGC111102 antibody, MGC8703 antibody, OTTHUMP00000210362 antibody, OTTHUMP00000210364 antibody, OTTHUMP00000210367 antibody, Paternally expressed gene 1 antibody, Paternally-expressed gene 1 protein antibody, PEG1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q5EB52
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: MEST

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

Images

Immunofluorescence staining of MCF-7 cells with CSB-PA704858ESR1HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA704858ESR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human Pancreatic tissue using CSB-PA704858ESR1HU at dilution of 1:50

Immunohistochemistry of paraffin-embedded human Breast cancer using CSB-PA704858ESR1HU at dilution of 1:50

Western blot
All lanes: MEST antibody at 5.78ug/ml + Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39, 38, 34 kDa
Observed band size: 39 kDa

Western Blot
Positive WB detected in: NTERA-2 whole cell lysate, U87 whole cell lysate, MCF7 whole cell lysate, HT29 whole cell lysate
All lanes: MEST antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 38, 34 kDa
Observed band size: 35 kDa