IL23R Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA733162LA01HU
Article Name: IL23R Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA733162LA01HU
Supplier Catalog Number: CSB-PA733162LA01HU
Alternative Catalog Number: CSB-PA733162LA01HU-100UL, CSB-PA733162LA01HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: IL 23R antibody, IL-23 receptor antibody, IL-23R antibody, IL23 Receptor antibody, Il23r antibody, IL23R_HUMAN antibody, Interleukin 23 receptor antibody, Interleukin-23 receptor antibody
Clonality: Polyclonal
UniProt: Q5VWK5
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: IL23R
Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:20-1:100
Immunofluorescence staining of MCF-7 cell with CSB-PA733162LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: K562 whole cell lysate(30µg), 293T whole cell lysate(30µg), Raji whole cell lysate(30µg)
All lanes: IL23R antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 72,43,41,20,30 kDa
Observed band size: 72 kDa
Exposure time: 120s