Clec4g Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA803912ZA01MO
- Images (8)
| Article Name: | Clec4g Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA803912ZA01MO |
| Supplier Catalog Number: | CSB-PA803912ZA01MO |
| Alternative Catalog Number: | CSB-PA803912ZA01MO-100UL, CSB-PA803912ZA01MO-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | C-type lectin domain family 4 member G, Clec4g |
| Clonality: | Polyclonal |
| UniProt: | Q8BNX1 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | Clec4g |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:30-1:100 |
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Immunofluorescence staining of Hela cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of NIH/3T3 cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of Hela cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of NIH/3T3 cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA803912ZA01MO diluted at 1:66 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-PA803912ZA01MO diluted at 1:66 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: Mouse Heart tissue lysate All lanes: Clec4g antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa |
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Western Blot Positive WB detected in: Mouse Heart tissue lysate All lanes: Clec4g antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa |
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