NECTIN4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: NECTIN4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA822274LA01HU
• Supplier Catalog Number: CSB-PA822274LA01HU
• Alternative Catalog Number: CSB-PA822274LA01HU-100UG, CSB-PA822274LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: DKFZp686K05193 antibody, EDSS1 antibody, Ig superfamily receptor LNIR antibody, Nectin 4 antibody, Nectin-4 antibody, poliovirus receptor-related 4 antibody, Processed poliovirus receptor-related protein 4 antibody, PRR4 antibody, pvrl4 antibody, PVRL4_HUMAN antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q96NY8
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: NECTIN4

Application Notes

• Application Dilute: Recommended dilution: IF:1:50-1:200

Images

Immunofluorescence staining of NIH/3T3 cell with CSB-PA822274LA01HU at 1:35, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of NIH/3T3 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA822274LA01HU diluted at 1:300 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in:A549 whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate, Mouse Spleen tissue lysate
All lanes:NECTIN4 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56kDa
Observed band size: 56 kDa