SLC7A6 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SLC7A6 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA849761LA01HU
• Supplier Catalog Number: CSB-PA849761LA01HU
• Alternative Catalog Number: CSB-PA849761LA01HU-100UG, CSB-PA849761LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: SLC7A6 antibody, KIAA0245 antibody, Y+L amino acid transporter 2 antibody, Cationic amino acid transporter antibody, y+ system antibody, Solute carrier family 7 member 6 antibody, y(+)L-type amino acid transporter 2 antibody, Y+LAT2 antibody, y+LAT-2 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q92536
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: SLC7A6

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200

Images

Imm

Immunofluorescence staining of Hela cell with CSB-PA849761LA01HU at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA849761LA01HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-PA849761LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse Heart tissue lysate
All lanes: SLC7A6 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa