GSDMA Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GSDMA Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA850415LA01HU
• Supplier Catalog Number: CSB-PA850415LA01HU
• Alternative Catalog Number: CSB-PA850415LA01HU-100UL, CSB-PA850415LA01HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: GSDMA antibody, GSDM antibody, GSDM1 antibody, FKSG9Gasdermin-A antibody, Gasdermin-1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q96QA5
• Buffer: Preservative: 0.05% NaN3<br />Constituents: PBS containing 40% glycerol,pH7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: GSDMA

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:3000 , IF:1:20-1:100

Images

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IH

Immunofluorescence staining of HepG2 cell with CSB-PA850415LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: COLO205 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), 293 whole cell lysate(30µg), JK whole cell lysate(30µg)
All lanes: GSDMA antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 60s