MOV10L1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MOV10L1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA871604LA01HU
• Supplier Catalog Number: CSB-PA871604LA01HU
• Alternative Catalog Number: CSB-PA871604LA01HU-100UG, CSB-PA871604LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: Cardiac helicase activated by MEF2 protein antibody, Cardiac specific RNA helicase antibody, CHAMP antibody, Csm antibody, M10L1_HUMAN antibody, Moloney leukemia virus 10 like protein 1 antibody, Moloney leukemia virus 10-like protein 1 antibody, MOV10 like 1 antibody, MOV10 like protein 1 antibody, MOV10-like protein 1 antibody, MOV10L1 antibody, Mov10l1, Moloney leukemia virus 10 like 1, homolog (mouse) antibody, Putative helicase Mov10l1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q9BXT6
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: MOV10L1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Images

Immunofluorescence staining of HepG2 cells with CSB-PA871604LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA871604LA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA871604LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate
All lanes: MOV10L1 antibody at 6.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 136, 38, 14, 130, 131 kDa
Observed band size: 136 kDa