POLDIP3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: POLDIP3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA871610LA01HU
• Supplier Catalog Number: CSB-PA871610LA01HU
• Alternative Catalog Number: CSB-PA871610LA01HU-100UG, CSB-PA871610LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: 46 kDa DNA polymerase delta interaction protein antibody, DNA polymerase delta interacting protein 3 antibody, KIAA1649 antibody, P46 antibody, PDIP3_HUMAN antibody, PDIP46 antibody, POLDIP3 antibody, Polymerase (DNA-directed), delta interacting protein 3 antibody, Polymerase delta interacting protein 46 antibody, Polymerase delta-interacting protein 3 antibody, RNA-binding protein P46 antibody, S6K1 Aly/REF-like target antibody, SKAR antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q9BY77
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: POLDIP3

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200, IP:1:200-1:2000

Images

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871610LA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate
All lanes: POLDIP3 antibody at 3.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 43 kDa
Observed band size: 43 kDa

Immunofluorescence staining of Hela cells with CSB-PA871610LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating POLDIP3 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA871610LA01HU in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA871610LA01HU (8ug) + Jurkat whole cell lysate (500ug)
Lane 3: Jurkat whole cell lysate (10ug)