GHITM Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GHITM Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA872487LA01HU
• Supplier Catalog Number: CSB-PA872487LA01HU
• Alternative Catalog Number: CSB-PA872487LA01HU-100UG, CSB-PA872487LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Rat
• Conjugation: Unconjugated
• Alternative Names: Dermal papilla derived protein 2 antibody, Dermal papilla-derived protein 2 antibody, DERP2 antibody, Ghitm antibody, GHITM_HUMAN antibody, Growth hormone inducible transmembrane protein antibody, Growth hormone-inducible transmembrane protein antibody, HSPC282 antibody, MICS1 antibody, Mitochondrial morphology and cristae structure 1 antibody, My021 antibody, PTD010 antibody, TMBIM5 antibody, Transmembrane BAX inhibitor motif containing 5 antibody, Transmembrane BAX inhibitor motif containing protein 5 antibody, Transmembrane BAX inhibitor motif-containing protein 5 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q9H3K2
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: GHITM

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:500

Images

Immunofluorescence staining of HepG2 cells with CSB-PA872487LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA872487LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA872487LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Rat brain tissue
All lanes: GHITM antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa