GOLPH3 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA872494LA01HU
- Images (5)
| Article Name: | GOLPH3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA872494LA01HU |
| Supplier Catalog Number: | CSB-PA872494LA01HU |
| Alternative Catalog Number: | CSB-PA872494LA01HU-100UG, CSB-PA872494LA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC, IP, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | Coat protein antibody, Coat protein GPP34 antibody, FLJ90675 antibody, Golgi associated protein antibody, Golgi peripheral membrane protein 1, 34 kDa antibody, Golgi phosphoprotein 3 (coat protein) antibody, Golgi phosphoprotein 3 antibody, Golgi protein antibody, GOLP3_HUMAN antibody, Golph3 antibody, GOPP1 antibody, GPP34 antibody, MIDAS antibody, Mitochondrial DNA absence factor antibody |
| Clonality: | Polyclonal |
| UniProt: | Q9H4A6 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | GOLPH3 |
| Application Dilute: | Recommended dilution: WB:1:500-1:3000, IHC:1:100-1:300, IP:1:1000-1:2000 |
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IHC image of CSB-PA872494LA01HU diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody |
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Immunoprecipitating GOLPH3 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA010605A0HU in Hela whole cell lysate Lane 2:CSB-PA872494LA01HU(7.98µg)+ Hela whole cell lysate(600µg) Lane 3: Hela whole cell lysate(30µg) For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/20000) |
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Western Blot Positive WB detected in: Hela whole cell lysate(20µg), MCF7 whole cell lysate(20µg) All lanes: GOLPH3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 120s |
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