MOB1A Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MOB1A Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA872519HA01HU
• Supplier Catalog Number: CSB-PA872519HA01HU
• Alternative Catalog Number: CSB-PA872519HA01HU-100UG, CSB-PA872519HA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: B1, Mps One Binder kinase activator like 1B (yeast) antibody, C2orf6 antibody, Chromosome 2 open reading frame 6 antibody, FLJ10788 antibody, FLJ11595 antibody, MATS1 antibody, MOB kinase activator 1A antibody, MOB1 antibody, Mob1 alpha antibody, Mob1 homolog 1B antibody, MOB1 Mps One Binder homolog A antibody, MOB1 Mps One Binder kinase activator like 1B antibody, Mob1A antibody, MOB4B antibody, Mob4B protein antibody, MOBK1B antibody, Mobkl1b antibody, MOL1B_HUMAN antibody, Mps one binder kinase activator like 1B antibody, Mps one binder kinase activator-like 1B antibody, Protein Mob4B antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q9H8S9
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: MOB1A

Application Notes

• Application Dilute: Recommended dilution: WB:1:2000-1:5000, IHC:1:100-1:1000, IF:1:200-1:500, IP1:200-1:2000

Images

Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA872519HA01HU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA872519HA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA872519HA01HU diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating MOB1A in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA872519HA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA872519HA01HU (5µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)

Western Blot
Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate
All lanes: MOB1A antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26, 17 kDa
Observed band size: 26 kDa