ZDHHC4 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ZDHHC4 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA873612LA01HU
• Supplier Catalog Number: CSB-PA873612LA01HU
• Alternative Catalog Number: CSB-PA873612LA01HU-100UG, CSB-PA873612LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: 1810021D01Rik antibody, 2900029I10Rik antibody, DC1 antibody, DHHC 4 antibody, DHHC-4 antibody, FLJ10479 antibody, MGC108810 antibody, OTTHUMP00000025197 antibody, OTTHUMP00000115925 antibody, OTTHUMP00000115926 antibody, OTTMUSP00000026321 antibody, OTTMUSP00000026322 antibody, Probable palmitoyltransferase ZDHHC4 antibody, ZDHC4_HUMAN antibody, ZDHHC4 antibody, Zinc finger DHHC domain containing protein 4 antibody, Zinc finger DHHC domain-containing protein 4 antibody, Zinc finger protein 374 antibody, ZNF374 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q9NPG8
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: ZDHHC4

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:50-1:200, IF:1:50-1:200

Images

Immunohistochemistry of paraffin-emb

Immunofluorescence staining of HepG2 cell with CSB-PA873612LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with CSB-PA873612LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA873612LA01HU diluted at 1:75 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: Hela whole cell lysate(20µg), HepG2 whole cell lysate(20µg), A549 whole cell lysate(20µg), HT29 whole cell lysate(20µg)
All lanes: ZDHHC4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 120s

Western Blot
Positive WB detected in:Mouse Liver tissue lysate(20µg)
All lanes: ZDHHC4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 60s