ABCG8 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA875651LA01HU
Article Name: ABCG8 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA875651LA01HU
Supplier Catalog Number: CSB-PA875651LA01HU
Alternative Catalog Number: CSB-PA875651LA01HU-100UG, CSB-PA875651LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: ABCG8 antibody, ABCG8_HUMAN antibody, ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody, ATP binding cassette sub family G member 8 antibody, ATP binding cassette, sub family G (WHITE), member 8 antibody, ATP-binding cassette sub-family G member 8 antibody, GBD4 antibody, MGC142217 antibody, Sterolin 2 antibody, Sterolin-2 antibody, STSL antibody
Clonality: Polyclonal
UniProt: Q9H221
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: ABCG8
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IF:1:300-1:500
Immunofluorescence staining of MCF-7 cell with CSB-PA875651LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with CSB-PA875651LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: Mouse Brain tissue lysate, Mouse Heart tissue lysate, Mouse Kidney tissue lysate, Rat Brain tissue lysate, Rat Heart tissue lysate
All lanes: ABCG8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 71 kDa
Observed band size: 76 kDa