Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA875672ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA875672ESR2HU at dilution of 1:100
Immunofluorescent analysis of A549 cells using CSB-PA875672ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating SAV1 in NIH/3T3 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA875672ESR2HU in NIH/3T3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA875672ESR2HU (5µg) + NIH/3T3 whole cell lysate (500µg) Lane 3: NIH/3T3 whole cell lysate (20µg)
Western Blot Positive WB detected in: 293T whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate All lanes: SAV1 antibody at 1.62µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
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