PACSIN3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: PACSIN3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA892456LA01HU
• Supplier Catalog Number: CSB-PA892456LA01HU
• Alternative Catalog Number: CSB-PA892456LA01HU-100UG, CSB-PA892456LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: Endophilin 1 antibody, Endophilin I antibody, FLJ17861 antibody, OTTHUMP00000234262 antibody, OTTHUMP00000234263 antibody, OTTHUMP00000234264 antibody, PACN3_HUMAN antibody, Pacsin3 antibody, Protein kinase C and casein kinase substrate in neurons 3 antibody, Protein kinase C and casein kinase substrate in neurons protein 3 antibody, SDPIII antibody, SH3 domain containing protein 6511 antibody, SH3 domain-containing protein 6511 antibody, syndapin III antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q9UKS6
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: PACSIN3

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200

Images

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA892456LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA892456LA01HU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA892456LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Mouse liver tissue
All lanes: PACSIN3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

Western Blot
Positive WB detected in: Rat liver tissue
All lanes: PACSIN3 antibody at 2.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa