Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purity:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form:
liquid
Target:
PTEN
Application Dilute:
WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200
Immunofluorescence staining of methanol-fixed Hela cells using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody.
Immunofluorescence staining of methanol-fixed MEFcells using PTEN (Phospho-Ser380/Thr382/Thr383)Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody.
Western blot analysis of extracts from Hela and C6 cells,treated with EGF or PMA, using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody.
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