APP (sabirnetug) Recombinant Monoclonal Antibody, Clone: [15A10], Unconjugated

Catalog Number: CSB-RA001950MA3HU
Article Name: APP (sabirnetug) Recombinant Monoclonal Antibody, Clone: [15A10], Unconjugated
Biozol Catalog Number: CSB-RA001950MA3HU
Supplier Catalog Number: CSB-RA001950MA3HU
Alternative Catalog Number: CSB-RA001950MA3HU-100UL, CSB-RA001950MA3HU-50UL
Manufacturer: Cusabio
Category: Antikörper
Application: ELISA, FC, IF
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: A4 antibody, A4_HUMAN antibody, AAA antibody, ABETA antibody, ABPP antibody, AICD-50 antibody, AICD-57 antibody, AICD-59 antibody, AID(50) antibody, AID(57) antibody, AID(59) antibody, Alzheimer disease amyloid protein antibody, Amyloid intracellular domain 50 antibody, Amyloid intracellular domain 57 antibody, Amyloid intracellular domain 59 antibody, APP antibody, APPI antibody, Beta amyloid protein 42 antibody, Beta APP42 antibody, Beta-APP40 antibody, Beta-APP42 antibody, C31 antibody, Cerebral vascular amyloid peptide antibody, CVAP antibody, Gamma-CTF(50) antibody, Gamma-CTF(57) antibody, Gamma-CTF(59) antibody, PN-II antibody, PreA4 antibody, Protease nexin-II antibody, S-APP-alpha antibody, S-APP-beta antibody, sabirnetug
Clonality: Monoclonal
Clone Designation: [15A10]
UniProt: P05067
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: Affinity-chromatography
Form: Liquid
Target: APP
Antibody Type: Recombinant Antibody
Application Dilute: Recommended dilution: IF:1:50-1:200, FC:1:50-1:200
Overlay Peak curve showing hela cells stained with CSB-RA001950MA3HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
ImmunofluorescencestainingofHelacellwithCSB-RA001950MA3HUat1:30,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasFITC-conjugatedAffiniPureGoatAnti-HumanIgG(H+L) .
ImmunofluorescencestainingofA549cellwithCSB-RA001950MA3HUat1:30,counter-stainedwithDAPI.Thecellswerefixedin4%formaldehydeandblockedin10%normalGoatSerum.Thecellswerethenincubatedwiththeantibodyovernightat4C.ThesecondaryantibodywasFITC-conjugatedAffiniPureGoatAnti-HumanIgG(H+L) .