CYP19A1 Recombinant Monoclonal Antibody, Clone: [1H1], Unconjugated, Rabbit

Product Overview

• Article Name: CYP19A1 Recombinant Monoclonal Antibody, Clone: [1H1], Unconjugated, Rabbit
• Biozol Catalog Number: CSB-RA006394A0HU
• Supplier Catalog Number: CSB-RA006394A0HU
• Alternative Catalog Number: CSB-RA006394A0HU-100UL, CSB-RA006394A0HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19A1, ARO1, CYAR, CYP19

Product Properties

• Clonality: Monoclonal
• Clone Designation: [1H1]
• UniProt: P11511
• Buffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Purity: Affinity-chromatography
• Form: Liquid
• Target: CYP19A1
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:20-1:200

Images

Immunofluorescence staining of Hela cell with CSB-RA006394A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

IHC image of CSB-RA006394A0HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: 293 whole cell lysate(20µg) , K562 whole cell lysate(20µg) , Hela whole cell lysate(20µg) , MCF-7 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg)
All lanes: CYP19A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time:120s