FLOT1 Recombinant Monoclonal Antibody, Clone: [4C8B6], Unconjugated

Product Overview

• Article Name: FLOT1 Recombinant Monoclonal Antibody, Clone: [4C8B6], Unconjugated
• Biozol Catalog Number: CSB-RA008727MA1HU
• Supplier Catalog Number: CSB-RA008727MA1HU
• Alternative Catalog Number: CSB-RA008727MA1HU-100UL, CSB-RA008727MA1HU-50UL
• Manufacturer: Cusabio
• Category: Antikörper
• Application: ELISA, FC, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: FLOT 1 antibody, FLOT1 antibody, FLOT1_HUMAN antibody, Flotillin-1 antibody, Flotillin1 antibody, Integral membrane component of caveolae antibody, Reggie 2 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [4C8B6]
• UniProt: O75955
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Affinity-chromatography
• Form: Liquid
• Target: FLOT1
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200, FC:1:50-1:200

Images

Overlay Peak curve showing A431 cells stained with CSB-RA008727MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of NIH/3T3 cell with CSB-RA008727MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific.

Immunofluorescence staining of Hela cell with CSB-RA008727MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific.

Western Blot
Positive WB detected in: HEK293 whole cell lysate(20µg) , U-251MG whole cell lysate(20µg) , HeLa whole cell lysate(20µg) , Raji whole cell lysate(20µg) , A549 whole cell lysate(20µg) , HepG2 whole cell lysate(20µg)
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time:1min

Western Blot
Positive WB detected in: Rat brain tissue lysate(20µg)
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47.4 kDa
Observed band size: 48 kDa
Exposure time: 2min