GFAP Recombinant Monoclonal Antibody, Clone: [6B12], Unconjugated, Mouse
Catalog Number:
CSB-RA009369MA1HU
- Images (6)
| Article Name: | GFAP Recombinant Monoclonal Antibody, Clone: [6B12], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-RA009369MA1HU |
| Supplier Catalog Number: | CSB-RA009369MA1HU |
| Alternative Catalog Number: | CSB-RA009369MA1HU-100UL, CSB-RA009369MA1HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | Glial fibrillary acidic protein (GFAP) , GFAP |
| Clonality: | Monoclonal |
| Clone Designation: | [6B12] |
| UniProt: | P14136 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | Affinity-chromatography |
| Form: | Liquid |
| Target: | GFAP |
| Antibody Type: | Recombinant Antibody |
| Application Dilute: | Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:20-1:200, FC:1:20-1:200 |
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Overlay Peak curve showing Jurkat cells stained with CSB-RA009369MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of SH-SY5Y cell with CSB-RA009369MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) . |
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Immunofluorescence staining of U87 cell with CSB-RA009369MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) . |
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IHC image of CSB-RA009369MA1HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-RA009369MA1HU diluted at 1:300 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: MCF7 whole cell lysate,Mouse Brain tissue lysate,Rat Brain tissue lysate All lanes: GFAP antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 55 kDa |
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