GSTM3 Recombinant Monoclonal Antibody, Clone: [2H9D7], Unconjugated

Product Overview

• Article Name: GSTM3 Recombinant Monoclonal Antibody, Clone: [2H9D7], Unconjugated
• Biozol Catalog Number: CSB-RA009982MA1HU
• Supplier Catalog Number: CSB-RA009982MA1HU
• Alternative Catalog Number: CSB-RA009982MA1HU-100UL, CSB-RA009982MA1HU-50UL
• Manufacturer: Cusabio
• Category: Antikörper
• Application: ELISA, FC, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: brain GST antibody, brain type mu glutathione S transferase antibody, glutathione S alkyltransferase M3 antibody, glutathione S aryltransferase M3 antibody, glutathione S transferase M3 (brain) antibody, glutathione S transferase M3 antibody, Glutathione S transferase Mu 3 antibody, glutathione S transferase, Mu 3 antibody, Glutathione S-transferase Mu 3 antibody, GST class mu 3 antibody, GST class-mu 3 antibody, GST5 antibody, GSTB antibody, Gstm3 antibody, GSTM3-3 antibody, GSTM3_HUMAN antibody, GTM3 antibody, hGSTM3-3 antibody, MGC3310 antibody, MGC3704 antibody, S (hydroxyalkyl) glutathione lyase M3 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [2H9D7]
• UniProt: P21266
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Affinity-chromatography
• Form: Liquid
• Target: GSTM3
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, FC:1:50-1:200

Images

Overlay Peak curve showing MCF-7 cells stained with CSB-RA009982MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of CSB-RA009982MA1HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA009982MA1HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: HeLa whole cell lysate(20µg) , THP-1 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) , U-251MG whole cell lysate(20µg) , Jurkat whole cell lysate(20µg) , HepG2 whole cell lysate(20µg) , A549 whole cell lysate(20µg)
All lanes: GSTM3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time:2min

Western Blot
Positive WB detected in: Mouse kidney tissue lysate(20µg) , Mouse liver tissue lysate(20µg) , Rat skin tissue lysate(20µg)
All lanes: GSTM3 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 26.6 kDa
Observed band size: 27 kDa
Exposure time: 2min