HIST1H3A Recombinant Monoclonal Antibody, Clone: [28H10], Unconjugated, Mouse

Product Overview

• Article Name: HIST1H3A Recombinant Monoclonal Antibody, Clone: [28H10], Unconjugated, Mouse
• Biozol Catalog Number: CSB-RA010418MA1HU
• Supplier Catalog Number: CSB-RA010418MA1HU
• Alternative Catalog Number: CSB-RA010418MA1HU-100UL, CSB-RA010418MA1HU-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l) , HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ

Product Properties

• Clonality: Monoclonal
• Clone Designation: [28H10]
• UniProt: P68431
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Affinity-chromatography
• Form: Liquid
• Target: HIST1H3A
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:20-1:200

Images

Immunofluorescence staining of HepG2 cell with CSB-RA010418MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) .

Immunofluorescence staining of Hela cell with CSB-RA010418MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) .

IHC image of CSB-RA010418MA1HU diluted at 1:300 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA010418MA1HU diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: MCF7 whole cell lysate,JK whole cell lysate,K562 whole cell lysate,HepG2 whole cell lysate
All lanes: HIST1H3A antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa