YWHAZ Recombinant Monoclonal Antibody, Clone: [19G7E9], Unconjugated

Product Overview

• Article Name: YWHAZ Recombinant Monoclonal Antibody, Clone: [19G7E9], Unconjugated
• Biozol Catalog Number: CSB-RA026293MA1HU
• Supplier Catalog Number: CSB-RA026293MA1HU
• Alternative Catalog Number: CSB-RA026293MA1HU-100UL, CSB-RA026293MA1HU-50UL
• Manufacturer: Cusabio
• Category: Antikörper
• Application: ELISA, FC, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: 14 3 3 delta antibody, 14 3 3 protein zeta/delta antibody, 14 3 3 protein/cytosolic phospholipase A2 antibody, 14 3 3 zeta antibody, 14-3-3 protein zeta/delta antibody, 1433Z_HUMAN antibody, Epididymis luminal protein 4 antibody, Epididymis secretory protein Li 3 antibody, HEL S 3 antibody, HEL4 antibody, KCIP-1 antibody, KCIP1 antibody, MGC111427 antibody, MGC126532 antibody, MGC138156 antibody, Phospholipase A2 antibody, Protein kinase C inhibitor protein 1 antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody, Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody, YWHAD antibody, YWHAZ antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [19G7E9]
• UniProt: P63104
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Affinity-chromatography
• Form: Liquid
• Target: YWHAZ
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200, FC:1:50-1:200

Images

Overlay Peak curve showing A431 cells stained with CSB-RA026293MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .

Immunofluorescence staining of MCF-7 cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .

Western Blot
Positive WB detected in: HEK293T whole cell lysate(20µg) , SH-SY5Y whole cell lysate(20µg) , Jurkat whole cell lysate(20µg) , NIH/3T3 whole cell lysate(20µg) , A549 whole cell lysate(20µg) , K562 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) , MCF-7 whole cell lysate(20µg) , HeLa whole cell lysate(20µg)
All lanes: YWHAZ antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time:30s

Western Blot
Positive WB detected in: Mouse brain tissue lysate(20µg) , Rat brain tissue lysate(20µg) , Mouse uterus tissue lysate(20µg) , Rat uterus tissue lysate(20µg)
All lanes: YWHAZ antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 27.7 kDa
Observed band size: 28 kDa
Exposure time: 2min