H2AFY Recombinant Monoclonal Antibody, Clone: [17G2], Unconjugated, Rabbit

Product Overview

• Article Name: H2AFY Recombinant Monoclonal Antibody, Clone: [17G2], Unconjugated, Rabbit
• Biozol Catalog Number: CSB-RA135386A0HU
• Supplier Catalog Number: CSB-RA135386A0HU
• Alternative Catalog Number: CSB-RA135386A0HU-100UL, CSB-RA135386A0HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, FC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: Core histone macro h2a.1 antibody, Core histone macro-H2A.1 antibody, H2A histone family member Y antibody, H2A.y antibody, H2A/y antibody, H2AF12M antibody, H2AFJ antibody, H2afy antibody, H2AY_HUMAN antibody, Histone H2A.Y antibody, Histone macroH2A1 antibody, Histone macroH2A1.1 antibody, Histone macroH2A1.2 antibody, Macroh2a1 antibody, MACROH2A1.1 antibody, MacroH2A1.2 antibody, Medulloblastoma antigen MU MB 50.205 antibody, Medulloblastoma antigen MU-MB-50.205 antibody, mH2a antibody, mH2A1 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [17G2]
• UniProt: O75367
• Buffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Purity: Affinity-chromatography
• Form: Liquid
• Target: H2AFY
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Images

Overlay Peak curve showing THP-1 cells stained with CSB-RA135386A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunofluorescence staining of A431 cell with CSB-RA135386A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-RA135386A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA135386A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: Histone mH2A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time:10s