ACVR2A&ACVR2B Recombinant Monoclonal Antibody, Clone: [7E5], Unconjugated

Product Overview

• Article Name: ACVR2A&ACVR2B Recombinant Monoclonal Antibody, Clone: [7E5], Unconjugated
• Biozol Catalog Number: CSB-RA623829MA1HU
• Supplier Catalog Number: CSB-RA623829MA1HU
• Alternative Catalog Number: CSB-RA623829MA1HU-100UL, CSB-RA623829MA1HU-50UL
• Manufacturer: Cusabio
• Category: Antikörper
• Application: ELISA, IF, IHC
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Activin A receptor type IIB antibody, Activin receptor type 2B antibody, Activin receptor type IIB antibody, Activin receptor type-2B antibody, ActR IIB antibody, ACTR-IIB antibody, ActRIIB antibody, ACVR 2B antibody, ACVR2B antibody, AVR2B_HUMAN antibody, HTX4 antibody, MGC116908 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [7E5]
• UniProt: P27037
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Affinity-chromatography
• Form: Liquid
• Target: ACVR2A&ACVR2B
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: ELISA:1:5000-1:50000, IHC:1:50-1:200, IF:1:50-1:200

Images

The Binding Activity of Human ACVR2A with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2A(CSB-MP001260HU1) at 2 µg/mL can bind Anti-ACVR2A recombinant antibody . The EC50 is 3.848-4.375 ng/mL.

The Binding Activity of Mouse ACVR2A with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse ACVR2A(CSB-MP001260MO1) at 2 µg/mL can bind Anti-ACVR2A recombinant antibody. The EC50 is 1.562-1.966 ng/mL.

The Binding Activity of Mouse Acvr2b with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Acvr2b(CSB-MP001261MO1) at 2 µg/mL can bind Anti-Acvr2b recombinant antibody. The EC50 is 3.560-4.235 ng/mL.

The Binding Activity of Human ACVR2B with Anti-ACVR2A&ACVR2B recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2B(CSB-MP623829HU) at 2 µg/mL can bind Anti-ACVR2B recombinant antibody. The EC50 is 2.983-3.438 ng/mL.

Immunofluorescence staining of SH-SY5Y cell with CSB-RA623829MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L) .

IHC image of CSB-RA623829MA1HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA623829MA1HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.