TMPRSS2 Recombinant Monoclonal Antibody, Clone: [5H2], Unconjugated, Rabbit

Product Overview

• Article Name: TMPRSS2 Recombinant Monoclonal Antibody, Clone: [5H2], Unconjugated, Rabbit
• Biozol Catalog Number: CSB-RA981161A0HU
• Supplier Catalog Number: CSB-RA981161A0HU
• Alternative Catalog Number: CSB-RA981161A0HU-100UL, CSB-RA981161A0HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: Transmembrane protease serine 2 (EC 3.4.21.-) (Serine protease 10) [Cleaved into: Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain], TMPRSS2, PRSS10

Product Properties

• Clonality: Monoclonal
• Clone Designation: [5H2]
• UniProt: O15393
• Buffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Purity: Affinity-chromatography
• Form: Liquid
• Target: TMPRSS2
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200

Images

IHC image of CSB-RA981161A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: PC-3 whole cell lysate(30µg) , HT-29 whole cell lysate(30µg) , NIH/3T3 whole cell lysate(30µg) , Rat colorectal tissue lysate(30µg)
All lanes: TMPRSS2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time:3min