Anti-Mouse IgG was produced by repeated immunization with mouse IgG F(c) fragment in goat.
Conjugation:
Unconjugated
Alternative Names:
Mouse IgG F(c) Antibody, Gt-a-Mouse F(c), Mouse F(c) in goat, Goat F(c) Secondary Antibody, goat anti-mouse IgG F(c) fragment antibody
Format:
IgG
Target Specificity:
IgG (Fc)
Cross-Adsorption (MinX):
no cross-adsorbtion
Anti-Mouse IgG F(c) antibody generated in goat detects specifically mouse IgG F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Recep
Clonality:
Polyclonal
Concentration:
10.0 mg/mL
Isotype:
Ig
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-Mouse IgG F(c) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
Form:
Lyophilized
Formula:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Anti-Mouse IgG F(c) antibody has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
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