rabbit F(ab)2 Anti-DOG IgG F(ab)2 fragment Antibody, rabbit F(ab)2 Anti-DOG IgG Fab2 fragment Antibody, rabbit Fab2 Anti Dog IgG Fab2
Format:
F(ab')2
Target Specificity:
IgG (F(ab')2)
Cross-Adsorption (MinX):
no cross-adsorbtion
F(ab)2 Anti-Dog IgG F(ab)2 Antibody generated in rabbit detects Dog F(ab)2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma
Clonality:
Polyclonal
Concentration:
10.0 mg/mL
Isotype:
Ig
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG, Dog IgG F(ab’)2 and Dog Serum. No reaction was observed against Dog IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
Form:
Lyophilized
Formula:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Dog
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
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