F(ab)2 Anti-Dog IgG F(ab)2 Fluorescein Antibody generated in rabbit detects Dog F(ab)2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secrete
Clonality:
Polyclonal
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Dog IgG, Dog IgG F(ab’)2 and Dog Serum. No reaction was observed against Dog IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,Azide/BSA free
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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