rabbit F(ab)2 Anti-GUINEA PIG IgG F(ab)2 fragment Antibody, rabbit F(ab)2 Anti-GUINEA PIG IgG Fab2 fragment Antibody, rabbit Fab2 Anti Guinea Pig IgG Fab2
Format:
F(ab')2
Target Specificity:
IgG (F(ab')2)
Cross-Adsorption (MinX):
no cross-adsorbtion
F(ab)2 Anti-Guinea Pig IgG F(ab)2 Antibody generated in rabbit detects Guinea Pig F(ab)2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secre
Clonality:
Polyclonal
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab’)2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,Azide/BSA free
Target:
Guinea Pig
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000-1:5,000, Western Blot Dilution: 1:2,000-1:10,000
Application Notes:
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
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