Rabbit F(ab)2 anti-Hamster (all) IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Rabbit F(ab)2 anti-Hamster (all) IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: DNA-SEC-182677
• Supplier Catalog Number: SEC-182677
• Alternative Catalog Number: DNA-SEC-182677
• Manufacturer: dianova
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Hamster (all)
• Immunogen: Hamster IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: rabbit F(ab)2 Anti-GOLDEN SYRIAN HAMSTER IgG Antibody, rabbit Fab2 Anti Golden Syrian Hamster IgG
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Description

F(ab)2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimen

Product Properties

• Clonality: Polyclonal
• Isotype: Ig
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Purity: This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
• Form: Lyophilized
• Formula: 20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,Azide/BSA free
• Target: Golden Syrian Hamster
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000-1:5,000, Western Blot Dilution: 1:2,000-1:10,000
• Application Notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

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