F(ab)2 Anti-Rat IgG F(ab)2 Fluorescein Antibody generated in rabbit detects Rat F(ab)2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secrete
Clonality:
Polyclonal
Concentration:
10.0 mg/mL
Isotype:
Ig
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
Form:
Lyophilized
Formula:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Thimerosal
F(ab')2 Anti-Rat IgG F(ab')2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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