Rabbit IgG anti-Cat IgG (F(ab)2)-Biotin, MinX none

Catalog Number: DNA-SEC-182772
Article Name: Rabbit IgG anti-Cat IgG (F(ab)2)-Biotin, MinX none
Biozol Catalog Number: DNA-SEC-182772
Supplier Catalog Number: SEC-182772
Alternative Catalog Number: DNA-SEC-182772
Manufacturer: dianova
Host: Rabbit
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Feline
Immunogen: Cat IgG F(ab)2 fragment
Conjugation: Biotin
Alternative Names: rabbit anti Cat IgG F(ab)2 Antibody biotin conjugate, rabbit anti Cat IgG Fab2 Antibody biotin conjugate
Format: IgG
Target Specificity: IgG (F(ab')2)
Cross-Adsorption (MinX): no cross-adsorbtion
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutina
Clonality: Polyclonal
Concentration: 2.0 mg/mL
Isotype: Ig
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Cat IgG, Cat IgG F(ab’)2 and Cat Serum.  No reaction was observed against Cat IgG F(c).
Form: Lyophilized
Formula: 20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target: Cat
Antibody Type: Secondary Antibody
Application Dilute: ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:40,000 to 1:160,000 of the reconstitution concentration is suggested for this product.