Anti-Human IgG F(ab)2 fragment was produced in rabbit by repeated immunization with human IgG F(ab)2 fragment.
Conjugation:
Unconjugated
Alternative Names:
F(ab)2 Human IgG Antibody, Rb-a-Human F(ab)2 IgG, F(ab)2 Human IgG Antibody in Rabbit, Pre-Adsorbed Human IgG Secondary Antibody
Format:
IgG
Target Specificity:
IgG (F(ab')2)
Cross-Adsorption (MinX):
Mouse
Anti-Human IgG F(ab)2 Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab)2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled co
Clonality:
Polyclonal
Concentration:
2.0mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.
Form:
Liquid (sterile filtered)
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Human
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:350,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:3,000 - 1:15,000
Application Notes:
Anti-Human IgG F(ab)2 antibody has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
* VAT and and shipping costs not included. Errors and price changes excepted
