Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well
Clonality:
Polyclonal
Concentration:
2.19 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
Form:
Liquid (sterile filtered)
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:25,000, FLISA Dilution: User Optimized, Flow Cytometry Dilution: User Optimized, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, IF Microscopy Dilution: User Optimized, Immunoprecipitation Dilution: User Optimized, Western Blot Diluti
Application Notes:
Anti-Mouse IgG affinity purified secondary antibody is generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse IgG secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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