Anti-Mouse IgM (mu chain) antibody generated in goat detects specifically mouse IgM (mu chain). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spl
Clonality:
Polyclonal
Concentration:
2.19 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-Mouse IgM (mu chain) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.
Form:
Liquid (sterile filtered)
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,400 - 1:40,400, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Anti-Mouse IgM (mu chain) has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
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