Anti-Mouse IgG subclass pan reactive Secondary Antibody was produced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Conjugation:
RPE
Alternative Names:
rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody phycoerythrin conjugation, rabbit anti-Mouse IgG PE conjugated Antibody
Format:
IgG
Target Specificity:
IgG (Fc-Fragment)
Cross-Adsorption (MinX):
no cross-adsorbtion
Anti-Mouse IgG Phycoerythrin Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobul
Clonality:
Polyclonal
Concentration:
0.5 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Anti-Mouse IgG subclass pan reactive Phycoerythrin Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
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