Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse IgG2a heavy chain. Anti-Mouse IgG2a is ideal for investigators involved in Serum Protein Component research.
Clonality:
Polyclonal
Concentration:
1.02 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. Specificity was confirmed by ELISA. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a, IgG).
Form:
Liquid (sterile filtered)
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:15,000 - 1:30,000, Immunohistochemistry Dilution: 1:2,000 - 1:10,000, Western Blot Dilution: 1:4,000 - 1:20,000
Application Notes:
Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
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