Anti-Mouse lambda (lambda chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse lambda light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is t
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), and anti-Rabbit Serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA.. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Form:
Liquid (sterile filtered)
Formula:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:2,000, Immunohistochemistry Dilution: 1:200 - 1:1,000, Western Blot Dilution: 1:500 - 1:2,500
Application Notes:
Anti-Mouse lambda Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
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